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Calretinin

货号 货期 规格 / 价格 询价

Calretinin

品牌

Swant

货号

rCRh10

产品分类

Recombinant Protein

研究领域

Calretinin

产品概述

Freeze in small aliquots (e.g. 1 or 10 ug) and keep at -80°C. Avoid repeated freezing and thawing.

形式

This recombinant protein has been purified by affinity chromatography on a NTA-resin This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption we suggest the following procedure: - Dilute 1 ul of the antiserum 7696 (SWant) against calretinin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). - Add 1 ug of the recombinant protein to 1 ml of the diluted antibody solution and mix well. - Incubate for at least 6 hours in the cold. - Apply to tissue-sections and incubate for 3 days. - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.

浓度

6-His human calretinin (recombinant) produced in Escherichia Coli.

纯度

Recombinant human

试剂准备

lyophilized (10 or 100 µg in 25, resp. 155 µl of: 10 mM Tris, 50 mM NaCl, 2 mMCaCl2, 1 mM EDTA, 1mM ß-mercaptoethanol, pH 7.4).

复溶

Schwaller B., Buchwald P., Blümcke I., Celio M.R. and Hunziker W. (1993) Characterization of a polyclonal antiserum against the purified human recombinant calcium-binding protein calretinin. Cell Calcium 14: 639-648.

产品描述

Purification: Affinity chromat. Lot No: 22

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数量

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